Clarke G N
Division of Pathology, The Royal Women's Hospital, Carlton, Victoria, Australia.
Hum Reprod. 1997 Jun;12(6):1184-7. doi: 10.1093/humrep/12.6.1184.
A simplified quantitative cervical mucus penetration test was developed and applied to the semen of 21 prospective donors in order to evaluate its performance. Analysis of the data using stepwise multiple regression revealed that the concentration of spermatozoa in mucus was related primarily to the concentration of progressive spermatozoa in the semen (63% of variance explained), and secondarily to the average path velocity of spermatozoa in semen (70% of variance explained by a model incorporating both variables). However, consistent with previous studies, the derived indices of penetration efficiency including the percentage successful collisions (PSC), percentage successful entries (PSE) and the motile density ratio (MDR) were not significantly correlated with any of the semen variables examined, although multiple regression did derive models which explained 27-31% of the variance in these three dependent variables. The strong correlation and correspondence between the PSC and the MDR suggests that the latter may provide a simplified index of penetration efficiency for routine clinical use and further research in this area.
开发了一种简化的定量宫颈黏液穿透试验，并将其应用于21名潜在供体的精液，以评估其性能。使用逐步多元回归对数据进行分析后发现，黏液中精子的浓度主要与精液中前向运动精子的浓度有关（解释了63%的方差），其次与精液中精子的平均路径速度有关（包含这两个变量的模型解释了70%的方差）。然而，与先前的研究一致，尽管多元回归确实得出了解释这三个因变量27%-31%方差的模型，但包括成功碰撞百分比（PSC）、成功进入百分比（PSE）和活动密度比（MDR）在内的穿透效率衍生指标与所检测的任何精液变量均无显著相关性。PSC与MDR之间的强相关性和对应关系表明，后者可能为常规临床应用和该领域的进一步研究提供一种简化的穿透效率指标。
