二氧化碳对新生大鼠氧诱导视网膜病变的影响。

The effect of carbon dioxide on oxygen-induced retinopathy in the neonatal rat.

作者信息

Holmes J M, Zhang S, Leske D A, Lanier W L

机构信息

Department of Ophthalmology, Mayo Clinic, Rochester, MN 55905, USA.

出版信息

Curr Eye Res. 1997 Jul;16(7):725-32. doi: 10.1076/ceyr.16.7.725.5054.

DOI:10.1076/ceyr.16.7.725.5054

PMID:9222092

Abstract

PURPOSE

Hypercarbia has been suggested as a risk factor for retinopathy of prematurity. We investigated the effect of raised inspired carbon dioxide on oxygen-induced retinopathy in the neonatal rat.

METHODS

Newborn rats raised in expanded litters (n = 25 each) were exposed to cycles of hyperoxia (80% O2) and hypoxia (10% O2 for 7 days, followed by room air recovery for 5 days. During cyclic oxygen exposure, 3 litters (n = 75) were exposed to 10% CO2 (PaCO2 78 mm Hg +/- 6; mean +/- SD) and 3 litters (n = 75) were exposed to 0.2% CO2 (PaCO2 45 mm Hg +/- 7). Animals were sacrificed on day 13 and retinae were analyzed using fluorescein perfusion and ADPase staining techniques.

RESULTS

Neovascularization occurred in 85% of rats exposed to high CO2 compared to 52% of rats exposed to low CO2 (p = 0.001). The severity of neovascularization, in clock hours, was also greater in the rats exposed to high CO2 (p < 0.001).

CONCLUSIONS

Exposure to high CO2 results in an increased incidence and severity of neovascularization in a rat model for oxygen-induced retinopathy. Our results support the suggestion that hypercarbia may be a risk factor for retinopathy of prematurity.

摘要

目的

高碳酸血症被认为是早产儿视网膜病变的一个危险因素。我们研究了吸入二氧化碳升高对新生大鼠氧诱导性视网膜病变的影响。

方法

将饲养在扩大窝中的新生大鼠（每组25只）暴露于高氧（80% O₂）和低氧（10% O₂，持续7天，随后在空气中恢复5天）循环中。在循环氧暴露期间，3窝（n = 75）暴露于10% CO₂（动脉血二氧化碳分压78 mmHg ± 6；均值 ± 标准差），3窝（n = 75）暴露于0.2% CO₂（动脉血二氧化碳分压45 mmHg ± 7）。在第13天处死动物，使用荧光素灌注和腺苷三磷酸酶染色技术分析视网膜。