嵌合抗HBx抗体在大肠杆菌中的重组与表达。
Reconstruction and expression of chimeric anti-HBx antibody in Escherichia coli.
作者信息
Zhou G, Liu K D, Tang Z Y, Chen Y H, Wu X F, Schroeder C H
机构信息
Liver Cancer Institute, Shanghai Medical University, People's Republic of China.
出版信息
J Cancer Res Clin Oncol. 1997;123(6):325-30. doi: 10.1007/BF01438308.
Abstract
The variable regions of murine monoclonal anti-HBx immunoglobulin and the constant region of human antibody were cloned by reverse transcript-polymerase chain reaction (RT-PCR). The heavy-chain and light-chain variable regions were connected and coexpressed with human constant region C-r3 and C-k3 in the reconstructed vector of E. coli. The products showed high specificity and binding ability with HBx. Which is closely associated with hepatocarcinogenesis. This makes it possible to humanize the mouse monoclonal antibodies and express the fusion protein in E.coli for potential radioimmunotherapy in patients with hepatocellular carcinoma.
摘要
通过逆转录聚合酶链反应（RT-PCR）克隆了鼠源单克隆抗-HBx免疫球蛋白的可变区和人抗体的恒定区。重链和轻链可变区在大肠杆菌重组载体中与人类恒定区C-r3和C-k3连接并共表达。产物显示出与HBx具有高特异性和结合能力。HBx与肝癌发生密切相关。这使得鼠源单克隆抗体人源化并在大肠杆菌中表达融合蛋白成为可能，用于肝细胞癌患者的潜在放射免疫治疗。
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