针对乙肝病毒X蛋白的单克隆抗体免疫球蛋白可变区的克隆、细菌合成及特性鉴定

Cloning, bacterial synthesis, and characterization of immunoglobulin variable regions of a monoclonal antibody specific for the hepatitis B virus X protein.

作者信息

zu Putlitz J, Skerra A, Schröder C H, Zentgraf H, Wands J R

机构信息

Molecular Hepatology Laboratory, Massachusetts General Hospital Cancer Center, Harvard Medical School, Charlestown 02129, USA.

出版信息

Gene. 1998 Oct 9;221(1):143-9. doi: 10.1016/s0378-1119(98)00419-3.

DOI:10.1016/s0378-1119(98)00419-3

PMID:9852959

Abstract

The nucleotide (nt) sequences encoding the variable regions of the heavy (H) and light (L) chains were determined for a murine monoclonal antibody, 12/231/93, which is specific for a linear epitope located between amino acids 90 and 102 of the hepatitis B virus (HBV) X protein (HBx). The variable (V) regions of the H and L chains were shown to belong to the mouse H chain subgroup II (C) and kappa L chain group III, respectively. The cloned variable region sequences were used for the production of a Fab fragment in Escherichia coli, which had binding activity for membrane immobilized recombinant HBx. These gene sequences may be useful for the study of HBx function in cells that will support HBV replication.

摘要

测定了针对乙型肝炎病毒（HBV）X蛋白（HBx）90至102位氨基酸之间线性表位的鼠单克隆抗体12/231/93重链（H）和轻链（L）可变区的核苷酸（nt）序列。H链和L链的可变（V）区分别属于小鼠H链亚组II（C）和κ轻链组III。克隆的可变区序列用于在大肠杆菌中生产Fab片段，该片段对膜固定化重组HBx具有结合活性。这些基因序列可能有助于在支持HBV复制的细胞中研究HBx的功能。