Li J, Tang Z Y, Liu K D
Liver Cancer Institute, Shanghai Medical University.
Zhonghua Zhong Liu Za Zhi. 1994 Jan;16(1):11-4.
A mouse monoclonal antibody directed against the protein of hepatitis B virus X open reading frame was prepared. After radiolabeled with sodium 131I-iodine and administration, imaging was performed with gamma camera and radioactivity of the tumor, organs, and blood were counted in a mini-gamma counter at day 1, 3, 5, 7 in nude mice bearing HCC xenografts, and the biodistribution and T: NT ratio was calculated. Selective accumulation was observed in tumor sites on day 3 and a clear image of tumor was shown in gamma camera on day 7. The image of tumor in the irrelevant IgG group was not obtained at any time. The tumor:blood ratio was 0.48 and tumor:liver was 1.2 on day 1, then increased to 1.5 and 4.5 on day 7 in experimental group, respectively. Specific mAb uptake by tumor was significantly greater than nonspecific IgG(P < 0.05) on day 7. The results were encouraging. The finding of this study indicates the possibility of using 131I-anti-HBx mAb to target cytostatic drugs to HCC.
制备了一种针对乙型肝炎病毒X开放阅读框蛋白的小鼠单克隆抗体。用131I-碘化钠进行放射性标记并给药后，在荷人肝癌异种移植瘤的裸鼠中于第1、3、5、7天用γ相机进行成像，并用微型γ计数器对肿瘤、器官和血液的放射性进行计数，计算生物分布和T:NT比值。第3天在肿瘤部位观察到选择性聚集，第7天在γ相机上显示出清晰的肿瘤图像。在任何时间均未在无关IgG组获得肿瘤图像。实验组第1天肿瘤:血液比值为0.48，肿瘤:肝脏比值为1.2，第7天分别增至1.5和4.5。第7天肿瘤对特异性单克隆抗体的摄取显著高于非特异性IgG（P<0.05）。结果令人鼓舞。本研究结果表明使用131I-抗HBx单克隆抗体将细胞毒性药物靶向肝癌的可能性。
