Almeida M T, Ramalho-Santos J, Oliveira C R, Pedroso de Lima M C
Department of Biochemistry, Faculty of Medicine, and Center for Neurosciences of Coimbra, University of Coimbra, Portugal.
Biochem Biophys Res Commun. 1997 Jul 9;236(1):184-8. doi: 10.1006/bbrc.1997.6928.
We have developed a model system, consisting of rat brain synaptic vesicles and rat brain synaptic plasma membrane vesicles, to study the fusion process associated with the exocytotic release of neurotransmitters. Our results show a significant increase in the extent of fusion when the reaction takes place in cytosol compared to that obtained when fusion is carried out in buffer. This effect is mediated by cytosolic proteins, although N-ethylmaleimide-sensitive factor does not play a role in fusion. We also registered an almost complete inhibition of fusion when synaptic vesicles were pre-incubated with botulinum toxin B, indicating that synaptobrevin plays an important role in the coalescence of membrane lipids of the interacting membranes.
我们开发了一个模型系统，由大鼠脑突触小泡和大鼠脑突触质膜小泡组成，用于研究与神经递质胞吐释放相关的融合过程。我们的结果表明，与在缓冲液中进行融合相比，当反应在胞质溶胶中发生时，融合程度显著增加。这种效应是由胞质蛋白介导的，尽管N-乙基马来酰亚胺敏感因子在融合过程中不起作用。我们还发现，当突触小泡与肉毒杆菌毒素B预孵育时，融合几乎完全受到抑制，这表明突触融合蛋白在相互作用膜的膜脂融合中起重要作用。
