脊髓灰质炎病毒RNA翻译对聚（rC）结合蛋白2的需求。

Requirement of poly(rC) binding protein 2 for translation of poliovirus RNA.

作者信息

Blyn L B, Towner J S, Semler B L, Ehrenfeld E

机构信息

Department of Molecular Biology and Biochemistry, University of California, Irvine 92697, USA.

出版信息

J Virol. 1997 Aug;71(8):6243-6. doi: 10.1128/JVI.71.8.6243-6246.1997.

DOI:10.1128/JVI.71.8.6243-6246.1997

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC191892/

Abstract

Poly(rC) binding protein 2 (PCBP2) is one of several cellular proteins that interact specifically with a major stem-loop domain in the poliovirus internal ribosome entry site. HeLa cell extracts subjected to stem-loop IV RNA affinity chromatography were depleted of all detectable PCBP2. Such extracts were unable to efficiently translate poliovirus RNA, although extracts recovered from control columns of matrix unlinked to RNA retained full translation activity. Both translation and production of infectious progeny virus were restored in the PCBP2-depleted extracts by addition of recombinant PCBP2, but not by PCBP1, which is a closely related member of the protein family. The data show that PCBP2 is an essential factor, which is required for efficient translation of poliovirus RNA in HeLa cells.

摘要

聚（rC）结合蛋白2（PCBP2）是几种能与脊髓灰质炎病毒内部核糖体进入位点的一个主要茎环结构域特异性相互作用的细胞蛋白之一。经茎环IV RNA亲和层析处理的HeLa细胞提取物中所有可检测到的PCBP2都被耗尽。这些提取物无法有效翻译脊髓灰质炎病毒RNA，而从与RNA无连接的基质对照柱中回收的提取物则保留了完整的翻译活性。通过添加重组PCBP2，而非该蛋白家族的密切相关成员PCBP1，可使PCBP2耗尽的提取物中的翻译和感染性子代病毒的产生得以恢复。数据表明，PCBP2是HeLa细胞中脊髓灰质炎病毒RNA有效翻译所必需的关键因子。

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