Poritz L S, Tilberg A F, Koltun W A
Department of Surgery, Pennsylvania State University Medical Center, Hershey 17033, USA.
J Surg Res. 1997 May;69(2):316-20. doi: 10.1006/jsre.1997.5056.
We have previously demonstrated an increase in lymphocyte function associated antigen-1 (LFA-1) expression in the native intestines of animals with graft versus host disease (GVHD) after small bowel transplantation (SBTx). The present study evaluated LFA-1 expression on peripheral blood lymphocytes (PBLs) during GVHD. GVHD was created in LBNF1 rats by heterotopic vascularized SBTx from Lewis donors and compared to sham-op controls and cyclosporine-treated SBTx rats (SBTx-CyA, 10 mg/kg/day). PBLs were harvested on Postop Day 13 when signs of severe GVHD were present and PBLs were stained for LFA-1, CD3 (T-cell marker), and CD45 (B cell marker) and analyzed on an Epics 5 flow cytometer. Mesenteric lymph node (MLN) lymphocytes from the native intestines were also harvested for each group and stained for LFA-1. There were significant decreases in LFA-1, CD3, and CD45 PBL expression in rats with GVHD. CyA treatment corrected the abnormal CD3 and CD45 ratios, but not LFA-1 expression. It is concluded that: (1) The proportion of PBLs expressing LFA-1 is depressed in animals with clinical GVHD consistent with their recruitment into the host's inflamed tissues. (2) CyA treatment corrects the abnormalities in T and B cell ratios but not the decreased expression of LFA-1 on PBLs and may relate to its known imperfect ability to treat GVHD.
我们之前已经证明，小肠移植（SBTx）后患有移植物抗宿主病（GVHD）的动物的天然肠道中淋巴细胞功能相关抗原-1（LFA-1）表达增加。本研究评估了GVHD期间外周血淋巴细胞（PBL）上LFA-1的表达。通过来自Lewis供体的异位血管化SBTx在LBNF1大鼠中诱导GVHD，并与假手术对照组和环孢素治疗的SBTx大鼠（SBTx-CyA，10mg/kg/天）进行比较。在术后第13天出现严重GVHD迹象时采集PBL，对PBL进行LFA-1、CD3（T细胞标志物）和CD45（B细胞标志物）染色，并在Epics 5流式细胞仪上进行分析。每组还采集天然肠道的肠系膜淋巴结（MLN）淋巴细胞并进行LFA-1染色。患有GVHD的大鼠中LFA-1、CD3和CD45 PBL表达显著降低。环孢素治疗纠正了异常的CD3和CD45比例，但未纠正LFA-1表达。得出以下结论：（1）在患有临床GVHD的动物中，表达LFA-1的PBL比例降低，这与其被募集到宿主的炎症组织中一致。（2）环孢素治疗纠正了T和B细胞比例的异常，但未纠正PBL上LFA-1表达的降低，这可能与其治疗GVHD的已知不完全能力有关。
