基于硅基微结构的聚合酶链反应性能优化。
Optimization of the performance of the polymerase chain reaction in silicon-based microstructures.
作者信息
Taylor T B, Winn-Deen E S, Picozza E, Woudenberg T M, Albin M
机构信息
PE Applied Biosystems, 850 Lincoln Centre Drive, Foster City, CA 94404, USA.
出版信息
Nucleic Acids Res. 1997 Aug 1;25(15):3164-8. doi: 10.1093/nar/25.15.3164.
Abstract
We have demonstrated the ability to perform real-time homogeneous, sequence specific detection of PCR products in silicon microstructures. Optimal design/ processing result in equivalent performance (yield and specificity) for high surface-to-volume silicon structures as compared to larger volume reactions in polypropylene tubes. Amplifications in volumes as small as 0.5 microl and thermal cycling times reduced as much as 5-fold from that of conventional systems have been demonstrated for the microstructures.
摘要
我们已经证明了在硅微结构中对PCR产物进行实时均相、序列特异性检测的能力。与聚丙烯管中较大体积的反应相比，优化的设计/处理使得高表面积与体积比的硅结构具有同等的性能（产量和特异性）。对于微结构，已经证明其扩增体积小至0.5微升，热循环时间比传统系统减少了多达5倍。
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