Mork Family Department of Chemical Engineering and Materials Science, 925 Bloom Walk, HED 216, Los Angeles, California 90089, United States.
Department of Chemistry, University of Southern California, 3620 McClintock Ave, SGM 418, Los Angeles, California 90089, United States.
ACS Appl Bio Mater. 2022 Feb 21;5(2):818-824. doi: 10.1021/acsabm.1c01180. Epub 2022 Feb 9.
3D printed microfluidics offer several advantages over conventional planar microfabrication techniques including fabrication of 3D microstructures, rapid prototyping, and inertness. While 3D printed materials have been studied for their biocompatibility in cell and tissue culture applications, their compatibility for in vitro biochemistry and molecular biology has not been systematically investigated. Here, we evaluate the compatibility of several common enzymatic reactions in the context of 3D-printed microfluidics: (1) polymerase chain reaction (PCR), (2) T7 in vitro transcription, (3) mammalian in vitro translation, and (4) reverse transcription. Surprisingly, all the materials tested significantly inhibit one or more of these in vitro enzymatic reactions. Inclusion of BSA mitigates only some of these inhibitory effects. Overall, inhibition appears to be due to a combination of the surface properties of the resins as well as soluble components (leachate) originating in the matrix.
3D 打印微流控技术相对于传统的平面微加工技术具有许多优势，包括制造 3D 微结构、快速原型制作和惰性。虽然已经研究了 3D 打印材料在细胞和组织培养应用中的生物相容性，但它们在体外生物化学和分子生物学方面的兼容性尚未得到系统研究。在这里，我们评估了几种常见酶促反应在 3D 打印微流控中的兼容性：（1）聚合酶链反应（PCR），（2）T7 体外转录，（3）哺乳动物体外翻译，以及（4）逆转录。令人惊讶的是，所有测试的材料都显著抑制了这些体外酶促反应中的一种或多种。BSA 的包含仅减轻了其中一些抑制作用。总体而言，抑制似乎是由于树脂的表面特性以及源自基质的可溶性成分（浸出物）的组合所致。
