Wilding P, Shoffner M A, Kricka L J
Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia 19104.
Clin Chem. 1994 Sep;40(9):1815-8.
Devices for performing polymerase chain reactions (PCR) have been developed for use with photolithographed silicon. Microchambers capable of holding between 5.0 and 10 microL of PCR reagents were constructed by etching specific areas of rectangular silicon chips (17 x 15 mm), which were then capped with Pyrex glass. These silicon devices (PCRChips), which were etched to depths of 40-80 microns, permitted free flow of fluids in the microchannels and microchambers. Access to the microchambers was through holes in the silicon. Thermal cycling of the PCR reagents was achieved by placing the disposable PCRChip in a small holder containing a computer-controlled Peltier heater-cooler. Successful amplification was demonstrated by electrophoresis of products in agarose gel containing ethidium bromide, and the migration of the product was compared with that obtained in a commercially available thermal cycler. The thermal characteristics of the silicon, coupled with the high surface area to volume ratio in the new devices, are particularly advantageous features for amplification by PCR.
用于进行聚合酶链反应（PCR）的设备已开发出来，可与光刻硅一起使用。通过蚀刻矩形硅芯片（17×15毫米）的特定区域构建了能够容纳5.0至10微升PCR试剂的微腔，然后用派热克斯玻璃覆盖。这些蚀刻深度为40 - 80微米的硅设备（PCR芯片）允许流体在微通道和微腔中自由流动。通过硅上的孔可进入微腔。将一次性PCR芯片放入装有计算机控制的珀耳帖加热器 - 冷却器的小支架中，实现PCR试剂的热循环。通过在含有溴化乙锭的琼脂糖凝胶中对产物进行电泳证明了成功扩增，并将产物的迁移情况与在市售热循环仪中获得的情况进行比较。硅的热特性，再加上新设备中高的表面积与体积比，是通过PCR进行扩增的特别有利的特征。
