大鼠上橄榄复合体中胆碱能传递标志物的密度测定评估

Densitometric evaluation of markers for cholinergic transmission in rat superior olivary complex.

作者信息

Yao W, Godfrey D A

机构信息

Department of Otolaryngology, Head and Neck Surgery, Medical College of Ohio, Toledo 43699-0008, USA.

出版信息

Neurosci Lett. 1997 Jun 20;229(1):21-4. doi: 10.1016/s0304-3940(97)00400-x.

DOI:10.1016/s0304-3940(97)00400-x

PMID:9224792

Abstract

Cholinergic transmission in the rat superior olivary complex (SOC) was assessed by densitometric measurements of labeling with several markers: acetylcholinesterase (AChE) histochemistry, choline acetyltransferase (ChAT) immunohistochemistry, and muscarinic acetylcholine receptor (M35) immunohistochemistry and N-methylscopolamine (NMS) binding autoradiography, as well as its subtype 2 (m2) immunohistochemistry. The markers which may occur in cholinergic neurons (ChAT, m2 receptor and AChE) showed dense labeling in the ventral nucleus of the trapezoid body (VNTB), in line with other evidence that it contains cholinergic neuron somata. The markers which are predominantly in cholinoceptive neurons (M35 and NMS) showed less labeling in the SOC, suggesting few muscarinic cholinergic inputs. These results are compared with those for the cochlear nucleus.

摘要

通过用几种标记物进行密度测量来评估大鼠上橄榄复合体（SOC）中的胆碱能传递：乙酰胆碱酯酶（AChE）组织化学、胆碱乙酰转移酶（ChAT）免疫组织化学、毒蕈碱型乙酰胆碱受体（M35）免疫组织化学和N-甲基东莨菪碱（NMS）结合放射自显影，以及其亚型2（m2）免疫组织化学。可能存在于胆碱能神经元中的标记物（ChAT、m2受体和AChE）在梯形体腹侧核（VNTB）中显示出密集标记，这与其他表明其含有胆碱能神经元胞体的证据一致。主要存在于胆碱感受神经元中的标记物（M35和NMS）在SOC中显示出较少的标记，表明毒蕈碱型胆碱能输入较少。将这些结果与耳蜗核的结果进行了比较。