Reid L L, Botta D, Lu Y, Gallagher E P, Kavanagh T J
Department of Environmental Health, University of Washington, Seattle, USA.
Biochim Biophys Acta. 1997 Jun 26;1352(3):233-7. doi: 10.1016/s0167-4781(97)00058-4.
Reverse transcription-polymerase chain reaction (RT-PCR) was used for the enzymatic synthesis of cDNA sequences encompassing the open reading frame for the catalytic subunit of mouse kidney glutamate-cysteine ligase (Glclc). Comparison of the mouse Glclc cDNA sequence and predicted protein sequence with that of rat Glclc and human GLCLC revealed between 94.8% and 88.4% cDNA homology and 98.4% to 95% amino acid identity, respectively.
