Wei W, Twell D, Lindsey K
Department of Biological Sciences, University of Durham, UK.
Plant J. 1997 Jun;11(6):1307-14. doi: 10.1046/j.1365-313x.1997.11061307.x.
A gene encoding a predicted nucleic acid helicase was identified in Arabidopsis thaliana by activation of a promoter trap. An in vivo transcriptional fusion between the helicase gene and a promoterless uidA (gusA) gene was generated, which was expressed specifically in the tapetum and vascular tissues. The tagged gene, designated HVT1 (Helicase in Vascular tissue and Tapetum), encodes a native transcript of approximately 4.4 kb, which is of very low abundance. The predicted HVT1 protein, of 1291 amino acid residues, is homologous to the Drosophila MALELESS, human RNA helicase A and bovine nuclear DNA helicase proteins, and represents the first identified member of a new subgroup within the mle group of the DEAH family. Low stringency genomic Southern blot analysis indicates that at least two structurally related genes exist in Arabidopsis. We suggest that the HVT1 protein may play a role in nucleic acid unwinding in restricted cell types during both vegetative and reproductive phases of the Arabidopsis life cycle.
通过启动子捕获在拟南芥中鉴定出一个编码预测核酸解旋酶的基因。构建了解旋酶基因与无启动子uidA（gusA）基因之间的体内转录融合体，该融合体在绒毡层和维管组织中特异性表达。标记的基因命名为HVT1（维管组织和绒毡层中的解旋酶），编码一个约4.4 kb的天然转录本，其丰度非常低。预测的HVT1蛋白有1291个氨基酸残基，与果蝇的MALELESS、人类RNA解旋酶A和牛核DNA解旋酶蛋白同源，是DEAH家族mle组内一个新亚组中首个被鉴定的成员。低严谨度基因组Southern杂交分析表明拟南芥中至少存在两个结构相关的基因。我们认为HVT1蛋白可能在拟南芥生命周期的营养和生殖阶段在特定细胞类型的核酸解旋中发挥作用。
