Iwasaki S, Iwabuchi Y, Asami T
Department of Dental Histology, Nippon Dental University School of Dentistry at Niigata, Niigata, Japan.
Tissue Cell. 1997 Jun;29(3):323-38. doi: 10.1016/s0040-8166(97)80008-0.
Comparative observations of the effects of cholinergic and adrenergic agonists (such as pilocarpine, phenylephrine and isoproterenol) on the secretion of salivary fluid and of secretory granules from the lingual epithelium and the lingual gland in the Tokyo Daruma pond frog, Rana porosa porosa, were made by light and transmission electron microscopy. The effect of pilocarpine on the loss of cytoplasm in both the lingual epithelium and the lingual gland was the strongest, and that of isoproterenol was the weakest. Transmission electron microscopy revealed that electron-dense granules, located in cells in both the lingual epithelium and the lingual gland, were discharged by exocytosis after stimulation by phenylephrine and isoproterenol. Immediately before secretion of these granules, they became somewhat larger and round or distorted in shape. However, after administration of pilocarpine, no obvious discharge of electron-dense granules was apparent and, instead, granules in some cells began deteriorating within the cells and those in other cells developed electron-dense and electron-lucent areas. A dot-like pattern was recognized in the electron-dense areas of these granules. These phenomena were assumed to be secondary effects accompanying the secretion of salivary fluid from cells of the lingual epithelium and the lingual gland. By contrast, mucous granules in the lingual gland were secreted by a holocrine process.
利用光学显微镜和透射电子显微镜，对胆碱能和肾上腺素能激动剂（如毛果芸香碱、去氧肾上腺素和异丙肾上腺素）对东京田蛙（Rana porosa porosa）舌上皮和舌腺唾液分泌及分泌颗粒的影响进行了比较观察。毛果芸香碱对舌上皮和舌腺细胞质丢失的影响最强，而异丙肾上腺素的影响最弱。透射电子显微镜显示，位于舌上皮和舌腺细胞中的电子致密颗粒，在去氧肾上腺素和异丙肾上腺素刺激后通过胞吐作用排出。在这些颗粒分泌之前，它们会变得稍大，形状呈圆形或扭曲。然而，在给予毛果芸香碱后，没有明显的电子致密颗粒排出，相反，一些细胞内的颗粒开始在细胞内退化，而其他细胞内的颗粒则出现电子致密区和电子透亮区。在这些颗粒的电子致密区可识别出点状图案。这些现象被认为是舌上皮和舌腺细胞分泌唾液时伴随的次要效应。相比之下，舌腺中的黏液颗粒通过全质分泌过程分泌。
