Smith P C, Mengeling W L
Can J Comp Med. 1977 Oct;41(4):364-8.
Heat-inactivated pseudorabies virus caused cutaneous delayed-type hypersensitive reactions when injected intradermally on the dorsolateral aspect of the thorax and tips of the ears of pigs previously exposed to the homologous virus. The reaction induced by subcutaneous injection in the lower eyelid was more easily administered and evaluated. Nonexposed control pigs did not react to the antigen and exposed and control pigs did not react when injected with a cell control antigen prepared in a similar manner. A positive response was detectable as early as seven days after exposure, reached near maximal levels by 28 days, and remained at similar levels for at least 90 days.
热灭活伪狂犬病病毒经皮内注射到先前接触过同源病毒的猪的胸外侧和耳尖时，会引起皮肤迟发型超敏反应。在下眼睑进行皮下注射诱导的反应更易于操作和评估。未接触过病毒的对照猪对抗原无反应，接触过病毒的猪和对照猪注射以类似方式制备的细胞对照抗原时也无反应。暴露后最早7天即可检测到阳性反应，28天左右达到接近最大水平，并在至少90天内保持在相似水平。
