Kelling C L, Staudinger W L, Rhodes M B
Am J Vet Res. 1982 Dec;43(12):2114-20.
Pseudorabies virus (PRV) antibodies, detectable by indirect radioimmunoassay (IRIA), serum-virus neutralization test (NT), or microimmunodiffusion test (MIDT) were developed within 8 days after pigs were inoculated with virulent PRV or attenuated PRV vaccine. Indirect radioimmunoassay and NT titers in pigs inoculated with virulent PRV were developed at the same rate, with IRIA titers being higher than NT titers. Pigs inoculated with attenuated or inactivated PRV vaccine developed peak mean prechallenge NT antibody titers of 4 and 1 (reciprocals of serum dilutions), respectively. Pigs inoculated with attenuated PRV vaccine had peak mean prechallenge IRIA antibody titers of 6, whereas pigs inoculated with inactivated PRV vaccine had mean IRIA antibody titers of 64. Challenge exposure of swine inoculated with attenuated or inactivated PRV vaccine elicited quantitatively equivalent responses, as measured by IRIA or NT, which were higher than prechallenge titers. There were no false-positive IRIA, NT, or MIDT results obtained when sera from nonvaccinated, nonchallenge-exposed pigs were tested. It appears that the PRV infection status of a seropositive swine herd could be ascertained by serologically monitoring several representative animals from a herd, using the NT. If 2 or more tests of representative animals at 14-day intervals were done and the mean NT titer was 4 or less, it could be concluded that the herd was vaccinated against, but not infected with, virulent virus.
猪接种强毒伪狂犬病病毒（PRV）或减毒PRV疫苗后8天内，可通过间接放射免疫测定（IRIA）、血清病毒中和试验（NT）或微量免疫扩散试验（MIDT）检测到PRV抗体。接种强毒PRV的猪，其间接放射免疫测定和NT效价以相同速率产生，IRIA效价高于NT效价。接种减毒或灭活PRV疫苗的猪，攻毒前NT抗体平均效价峰值分别为4和1（血清稀释倍数的倒数）。接种减毒PRV疫苗的猪，攻毒前IRIA抗体平均效价峰值为6，而接种灭活PRV疫苗的猪，IRIA抗体平均效价为64。用IRIA或NT检测时，接种减毒或灭活PRV疫苗的猪经攻毒暴露后产生的反应在数量上相当，且高于攻毒前效价。对未接种疫苗、未攻毒暴露的猪的血清进行检测时，未获得IRIA、NT或MIDT假阳性结果。似乎通过使用NT对猪群中的几只代表性动物进行血清学监测，可以确定血清阳性猪群的PRV感染状况。如果每隔14天对代表性动物进行2次或更多次检测，且平均NT效价为4或更低，则可以得出该猪群已接种疫苗但未感染强毒病毒的结论。
