Gupta R, Webster C I, Walker A R, Gray J C
Department of Plant Sciences, University of Cambridge, UK.
Plant Mol Biol. 1997 Jun;34(3):529-36. doi: 10.1023/a:1005828430861.
A cDNA encoding the HMG-I/Y protein from Arabidopsis thaliana has been isolated and characterised by nucleotide sequencing. The 903 bp cDNA contains a 612 bp open reading frame encoding a protein of 204 amino acid residues showing homology to HMG-I/Y proteins from other plant species. The protein contains four copies of the 'AT-hook' motif which is involved in binding A/T-rich DNA. Southern blotting showed that the HMG-I/Y gene was present in a single copy in the Arabidopsis genome. The gene was localised to the top of chromosome 1 by RFLP analysis of F8 recombinant inbred lines. Northern blotting showed that the gene was expressed in all organs examined, with the highest expression in flowers and developing siliques.
已从拟南芥中分离出编码HMG-I/Y蛋白的cDNA，并通过核苷酸测序对其进行了表征。这个903 bp的cDNA包含一个612 bp的开放阅读框，编码一个由204个氨基酸残基组成的蛋白质，该蛋白质与其他植物物种的HMG-I/Y蛋白具有同源性。该蛋白质包含四个“AT-钩”基序拷贝，这些基序参与结合富含A/T的DNA。Southern杂交显示HMG-I/Y基因在拟南芥基因组中以单拷贝形式存在。通过对F8重组自交系的RFLP分析，该基因被定位到第1号染色体的顶端。Northern杂交显示该基因在所有检测的器官中均有表达，在花和发育中的角果中表达最高。
