Yamamoto S, Minamikawa T
Department of Biology, Tokyo Metropolitan University, Japan.
Plant Mol Biol. 1997 Feb;33(3):537-44. doi: 10.1023/a:1005791728975.
Two cDNAs encoding high-mobility-group (HMG) proteins that correspond to animal HMG-Y proteins were isolated from maturing seeds of Canavalia gladiata D.C. The deduced amino acid sequences of these cDNAs showed similarity to other plant HMG-I/Y proteins reported to date. The mRNAs for the HMG-Y proteins were detected in leaves, stems, roots, pods and seeds of C. gladiata. The level of the mRNA was high in the maturing seeds of 30 days after flowering and 2-day germinated seeds. Two genomic clones were isolated from DNA of C. gladiata and both were shown to represent single-copy genes consisting of two exons and one intron. This is the first report of the genomic sequences for HMG-I/Y protein in plants.
从刀豆成熟种子中分离出两个编码与动物HMG-Y蛋白相对应的高迁移率族（HMG）蛋白的cDNA。这些cDNA推导的氨基酸序列与迄今报道的其他植物HMG-I/Y蛋白具有相似性。在刀豆的叶、茎、根、豆荚和种子中检测到HMG-Y蛋白的mRNA。在开花后30天的成熟种子和萌发2天的种子中，mRNA水平较高。从刀豆DNA中分离出两个基因组克隆，二者均显示为包含两个外显子和一个内含子的单拷贝基因。这是植物中HMG-I/Y蛋白基因组序列的首次报道。
