Latgé J P, De Bièvre C
Can J Microbiol. 1977 Oct;23(10):1340-5.
Changes in the relative abundance of cell constituents of Entomophthora virulenta Hall & Dunn were studied during the various differentiation phases leading to the formation of zygospores. The highest total protein (43%) and nucleic acid (5.2% RNA and 0.2% DNA) concentrations were found in the exponential growth phase; these concentrations decreased regularly as the mycelium aged. The amount of lipids was four times larger at the prespore and spore stage (about 25%) than at the exponential mycelial stage. The zygospore contained a very high concentration of chitin (about 17%), three times more than the mycelial concentrations. However, the percentage of polysaccharides without chitin, but including glycogen, was higher in the deceleration phase mycelium (34%) than in the spore (25%). A physiological model of the sexual sporulation of E. virulenta based on these data has been presented.
在导致接合孢子形成的各个分化阶段，对毒 Entomophthora virulenta Hall & Dunn 细胞成分的相对丰度变化进行了研究。在指数生长期发现总蛋白浓度最高（43%），核酸浓度最高（RNA 为 5.2%，DNA 为 0.2%）；随着菌丝体老化，这些浓度有规律地下降。在芽孢前和芽孢阶段，脂质含量（约 25%）是指数期菌丝体阶段的四倍。接合孢子含有非常高浓度的几丁质（约 17%），是菌丝体浓度的三倍。然而，不含几丁质但包括糖原的多糖百分比在减速期菌丝体中（34%）高于孢子中（25%）。基于这些数据，提出了毒 Entomophthora virulenta 有性孢子形成的生理模型。
