Latgé J P
Can J Microbiol. 1980 Sep;26(9):1038-48. doi: 10.1139/m80-176.
Growth and production of azygospores of Entomophthora obscura were studied through batch cultures in media containing glucose, or vegetable oil, and yeast extract. In media containing 3% glucose and 1% yeast extract, an 8-h lag phase occurred and sporulation began only at the 40th hour of culture; spore maturation lasted 4 days in average. Young mycelial stages were characterized by high nucleic acid and protein but low lipid concentrations. During sporogenesis stages, the quantity of lipids and chitin increased and the concentration of total polyosides (chitin excluded) was lower in comparison with the mycelial stages. Sporulation was induced by means of medium starvation in carbon and (or) nitrogen. The only sporulation inhibitor which allowed considerable growth was sodium fluoroacetate.
通过在含有葡萄糖、植物油和酵母提取物的培养基中进行分批培养，研究了暗绿虫霉接合孢子的生长和产生。在含有3%葡萄糖和1%酵母提取物的培养基中，出现了8小时的延迟期，仅在培养的第40小时开始产孢；孢子成熟平均持续4天。年轻的菌丝阶段的特点是核酸和蛋白质含量高，但脂质浓度低。在孢子发生阶段，脂质和几丁质的数量增加，与菌丝阶段相比，总多糖（不包括几丁质）的浓度较低。通过培养基中碳和（或）氮的饥饿诱导产孢。唯一允许大量生长的产孢抑制剂是氟乙酸钠。
