Micellar electrokinetic capillary chromatography of benzodiazepine antiepileptics and their desmethyl metabolites in blood.

A fast and reliable method for the MEKC separation and determination of benzodiazepine antipileptics and their active metabolites in serum has been developed, using a separation buffer composed of borate (pH 9.5), SDS (18 mM), and acetonitrile (14%) as an organic modifier. The method is sensitive enough to be used clinically with a precision of less than 3% for the analysis of nitrazepam, clonazepam, clobazam, diazepam and their desmethyl metabolites in serum.