Vincent-Naulleau S, Thibault D, Neway T, Pilet C
Institut d'Immunologie Animale et Comparée, Ecole Nationale Vétérinaire d'Alfort, Maisons-Alfort, France.
Res Immunol. 1997 Feb;148(2):127-36. doi: 10.1016/s0923-2494(97)82484-4.
The effects of polar glycopeptidolipids of Mycobacterium chelonae (pGPL-Mc) on haematopoietic stem cells and on megakaryocyte progenitors in bone marrow (BM) and spleen were investigated in mice. We studied the in vivo spleen colony-forming ability and marrow repopulating ability of pGPL-Mc by assays of colony-forming units-spleen (CFU-S). The number of CFU-S was increased in BM when both donors and recipients were treated with pGPL-Mc. In contrast, a single treatment of donors induced enhancement of spleen CFU-S. The number of pre-CFU-S was not significantly increased by pGPL-Mc injection. Megakaryocyte (Meg) progenitors were determined in vitro with a quantitative cultural analysis of bone marrow and spleen cells in agar in the presence of spleen-conditioned medium. A statistically significant increase in BM and spleen CFU-Meg was observed two days after the last administration of pGPL-Mc. This experiment points out the ability of pGPL-Mc to induce substantial stimulation of megakaryocytopoiesis and slight proliferation of stem cells in BM, but which is more pronounced in spleen. This molecule therefore appears to be a potential adjuvant of chemo- and radiotherapy in order to palliate the cytotoxic side effects of these cancer therapeutic modalities.
研究了龟分枝杆菌的极性糖肽脂（pGPL-Mc）对小鼠造血干细胞以及骨髓（BM）和脾脏中巨核细胞祖细胞的影响。我们通过脾集落形成单位（CFU-S）测定，研究了pGPL-Mc在体内的脾集落形成能力和骨髓重建能力。当供体和受体均用pGPL-Mc处理时，BM中的CFU-S数量增加。相反，对供体进行单次处理可增强脾脏CFU-S。注射pGPL-Mc后，前CFU-S的数量没有显著增加。在脾条件培养基存在下，通过对骨髓和脾细胞在琼脂中的定量培养分析，在体外测定巨核细胞（Meg）祖细胞。在最后一次施用pGPL-Mc两天后，观察到BM和脾脏CFU-Meg有统计学意义的增加。该实验指出，pGPL-Mc能够诱导BM中巨核细胞生成的大量刺激和干细胞的轻微增殖，但在脾脏中更为明显。因此，该分子似乎是化疗和放疗的潜在佐剂，以减轻这些癌症治疗方式的细胞毒性副作用。
