Tesfaye M, Holl F B
Faculty of Agricultural Sciences, University of British Columbia, Vancouver, Canada.
Can J Microbiol. 1998 Nov;44(11):1102-5.
Two 20-bp primers that provide group-specific detection of Rhizobium spp. by polymerase chain reaction (PCR) have been used to differentiate strains that belong to different effectiveness groups within the Rhizobium-Trifolium cross-inoculation group. The target for DNA amplification was a 370-bp fragment of the 23S rDNA region. Analysis of additional root-nodule forming, as well as root-associated bacterial species by PCR-primer assay revealed that variability within this 20-bp segment of the 23S rDNA region may be widespread and provide an effective identification tool. Our data suggest that strains of Rhizobium isolated from the perennial clover Trifolium semipilosum may be phylogenetically more closely related to Rhizobium etli.
两种20个碱基对的引物可通过聚合酶链反应（PCR）对根瘤菌属进行组特异性检测，已用于区分根瘤菌-三叶草交叉接种组内不同有效性组的菌株。DNA扩增的靶标是23S rDNA区域的一个370个碱基对的片段。通过PCR引物分析对其他形成根瘤的细菌以及根相关细菌物种进行分析，结果显示23S rDNA区域的这一20个碱基对片段内的变异性可能很普遍，并提供了一种有效的鉴定工具。我们的数据表明，从多年生三叶草半毛三叶草中分离出的根瘤菌菌株在系统发育上可能与埃氏根瘤菌关系更密切。
