Register K B, Boisvert A, Ackermann M R
Avian and Swine Respiratory Diseases Research Unit, USDA Agricultural Research Service, Ames, Iowa, USA.
Int J Syst Bacteriol. 1997 Jul;47(3):678-83. doi: 10.1099/00207713-47-3-678.
A total of 113 Bordetella bronchiseptica strains, isolated from 11 different host species worldwide, were characterized by ribotyping with restriction enzyme PvuII. Sixteen distinct ribotypes were identified, and each ribotype contained five to seven restriction fragments ranging in size from 1.8 to 5.6 kb. Approximately 88% of the swine isoaltes were identified as ribotype 3 strains. Isolates from dogs also displayed little variation; 74.1% were found to be ribotype 4 strains. Strains obtained from the remaining nine host species belonged to 15 different ribotypes. There was no association between geographic location and ribotype. The technique which we used may be useful for epidemiologic studies in which the transmission of B. bronchiseptica, both within and between species, is investigated.
对从全球11种不同宿主物种中分离出的总共113株支气管败血波氏杆菌菌株，用限制性内切酶PvuII进行核糖分型鉴定。共鉴定出16种不同的核糖型，每种核糖型包含5至7个大小在1.8至5.6 kb之间的限制性片段。大约88%的猪分离株被鉴定为核糖型3菌株。犬的分离株也显示出很少的变异；74.1%被发现是核糖型4菌株。从其余9种宿主物种获得的菌株属于15种不同的核糖型。地理位置与核糖型之间没有关联。我们所使用的这项技术可能对研究支气管败血波氏杆菌在物种内部和物种之间传播的流行病学研究有用。
