Register Karen B
Respiratory Diseases of Livestock Research Unit, USDA/Agricultural Research Service/National Animal Disease Center, P.O. Box 70, Ames, IA 50010, USA.
Vaccine. 2004 Nov 15;23(1):48-57. doi: 10.1016/j.vaccine.2004.07.020.
The product of the Bordetella bronchiseptica pertactin gene, prn, has been implicated as an adhesin and a protective immunogen in swine. Recent studies demonstrate prn sequence heterogeneity in swine isolates and vaccine strains within and surrounding the region 1 amino acid repeat GGXXP(n) and the region 2 amino acid repeat PQP(n). However, only a few isolates have been evaluated. Allelic variation between vaccine strains and field isolates may affect vaccine efficacy, since region 2 is known to encode an immunodominant protective epitope. In the present study, the DNA and predicted amino acid sequences of the pertactin repeat regions from a collection of 81 recent swine field isolates and 5 vaccine strains from the United States were determined. Two region 1 variants and four region 2 variants, one of which has not been previously reported, were identified, comprising four pertactin types. Four vaccines are derived from strains with a region 1 variant identical to that found in the majority of field isolates. However, only two vaccines possess the most commonly identified sequence in region 2, while two others contain a variant found in only one other swine isolate. Ribotype analysis demonstrated that although vaccines containing the novel region 2 variant fall within the same major cluster as other common swine ribotypes, they are less closely related. No relationship was observed between pertactin type and ribotype.
支气管败血波氏杆菌黏附素基因（prn）的产物已被认为是猪体内的一种黏附素和保护性免疫原。最近的研究表明，在猪分离株以及疫苗株中，1型氨基酸重复序列GGXXP(n)和2型氨基酸重复序列PQP(n)及其周围区域的prn序列存在异质性。然而，仅评估了少数分离株。疫苗株与田间分离株之间的等位基因变异可能会影响疫苗效力，因为已知2区编码一个免疫显性保护性表位。在本研究中，测定了来自美国的81株近期猪田间分离株和5株疫苗株的黏附素重复区域的DNA和预测氨基酸序列。鉴定出了2种1区变体和4种2区变体，其中一种此前未曾报道过，共构成4种黏附素类型。4种疫苗来源于1区变体与大多数田间分离株中相同的菌株。然而，只有2种疫苗在2区具有最常见的序列，另外2种含有仅在另一株猪分离株中发现的变体。核糖分型分析表明，虽然含有新型2区变体的疫苗与其他常见猪核糖型属于同一主要聚类，但它们的亲缘关系较远。未观察到黏附素类型与核糖型之间的关系。
