Surai P F, Kutz E, Wishart G J, Noble R C, Speake B K
Department of Physiology, Biochemistry and Nutrition, Poultry Research Institute, Borky, Kharkov Region, Ukraine.
J Reprod Fertil. 1997 May;110(1):47-51. doi: 10.1530/jrf.0.1100047.
This study is an attempt to enhance the resistance of chicken semen to peroxidative damage by supplementing the diet of cockerels with the major lipid-soluble antioxidant alpha-tocopherol. Cockerels at 6 months of age were fed for 8 weeks with feed containing 0, 20, 200 or 1000 mg alpha-tocopherol kg-1. Semen was collected during the final 2 weeks of the supplementation period and the concentrations of alpha-tocopherol in the spermatozoa and the seminal plasma were determined. The concentrations of alpha-tocopherol in whole semen, spermatozoa and seminal plasma were approximately twice as high when the supplementation was 200 mg kg-1 compared with when supplementation was 20 mg kg-1; however, supplementation at 1000 mg kg-1 did not achieve any further increase in these concentrations of alpha-tocopherol. Thus, the concentration of alpha-tocopherol in semen displays only a limited responsiveness to manipulation by dietary means. In contrast, the concentrations of the vitamin in the testes and liver were found to be much more amenable to dietary manipulation, exhibiting increases of six-seven-fold over the whole range of supplementation. However, the dietary-induced increase in the alpha-tocopherol content of semen did result in a significant reduction in the susceptibility of the semen to lipid peroxidation. A further effect of enhancing the concentration of this vitamin in the semen was a significant increase in the proportions of C20-22 polyunsaturated fatty acids in the sperm phospholipids. In addition, the proportion of phosphatidylethanolamine in the phospholipid was increased whereas that of sphingomyelin was reduced at the higher concentrations of alpha-tocopherol supplementation. Thus, an increased dietary intake of alpha-tocopherol does produce beneficial changes in the antioxidant capacity and lipid profile of semen, albeit to a relatively limited extent.
本研究旨在通过在公鸡日粮中添加主要的脂溶性抗氧化剂α-生育酚，提高鸡精液对过氧化损伤的抵抗力。对6月龄公鸡饲喂含0、20、200或1000 mg α-生育酚/千克的饲料，为期8周。在补充期的最后2周采集精液，并测定精子和精浆中α-生育酚的浓度。与补充20 mg/千克相比，补充200 mg/千克时，全精液、精子和精浆中α-生育酚的浓度约高出一倍；然而，补充1000 mg/千克并未使这些α-生育酚浓度进一步升高。因此，精液中α-生育酚的浓度对日粮调控的反应有限。相比之下，发现睾丸和肝脏中该维生素的浓度更易于通过日粮调控，在整个补充范围内呈现出6至7倍的增加。然而，日粮诱导的精液中α-生育酚含量增加确实导致精液对脂质过氧化的敏感性显著降低。提高精液中该维生素浓度的另一个作用是精子磷脂中C20 - 22多不饱和脂肪酸的比例显著增加。此外，在较高的α-生育酚补充浓度下，磷脂中磷脂酰乙醇胺的比例增加，而鞘磷脂的比例降低。因此，增加日粮中α-生育酚的摄入量确实会使精液的抗氧化能力和脂质谱产生有益变化，尽管程度相对有限。
