Ikenishi K, Tanaka T S
Department of Biology, Faculty of Science, Osaka City University, Japan.
Dev Growth Differ. 1997 Jun;39(3):345-9. doi: 10.1046/j.1440-169x.1997.t01-2-00010.x.
The number of primordial germ cells (PGC) in albino tadpoles of Xenopus is significantly decreased as compared with that of the wild-type. Whether the decreased number of PGC is caused by the presumptive PGC (pPGC) themselves or the micro-environment surrounding those cells in the albino, or both was investigated in the present study. [3H]thymidine-labeled pPGC of wild-type and albino were implanted into unlabeled, host neurulae of wild-type or albino and wild-type, respectively. Labeled PGC in the genital ridges of experimental tadpoles were examined by autoradiography. There were no significant differences in the proportion of tadpoles with labeled PGC and in the average number of those PGC between the albino and wild-type tadpoles, into which wild-type pPGC had been implanted. The proportion in wild-type tadpoles with albino pPGC was much lower than that in wild-type tadpoles with wild-type pPGC. These results suggest that the pPGC of the albino and not the micro-environment are responsible for the decreased number of PGC.
与野生型相比，非洲爪蟾白化蝌蚪中的原始生殖细胞（PGC）数量显著减少。本研究探讨了PGC数量减少是由白化蝌蚪中假定的原始生殖细胞（pPGC）自身、这些细胞周围的微环境，还是两者共同导致的。将野生型和白化型的[³H]胸腺嘧啶标记的pPGC分别植入未标记的野生型或白化型宿主神经胚以及野生型宿主神经胚中。通过放射自显影检查实验蝌蚪生殖嵴中标记的PGC。植入野生型pPGC的白化蝌蚪和野生型蝌蚪中，有标记PGC的蝌蚪比例以及这些PGC的平均数量没有显著差异。植入白化型pPGC的野生型蝌蚪中的比例远低于植入野生型pPGC的野生型蝌蚪中的比例。这些结果表明，是白化型的pPGC而非微环境导致了PGC数量的减少。
