Ikenishi K, Tanaka T S
Department of Biology, Faculty of Science, Osaka City University, Sumiyoshi, Japan.
Dev Growth Differ. 1997 Oct;39(5):625-33. doi: 10.1046/j.1440-169x.1997.t01-4-00010.x.
In order to understand the role of the protein of Xenopus vasa homolog (Xenopus vasa-like gene 1, XVLG1) in germ line cells, an attempt was made to perturb the function of the protein with the anti-vasa antibody 2L-13. The 2L-13 or the control antibody was microinjected with a lineage tracer (FITC-dextran-lysine, FDL) into single vegetal blastomeres containing the germ plasm of Xenopus 32-cell embryos, the descendants of which were destined to differentiate into a small number of primordial germ cells (PGC) and a large number of somatic cells, mostly of endodermal tissues at the tadpole stage. No significant effect of the injection of the antibodies on FDL-labeled, presumptive PGC (pPGC) was observed in embryos until stage 37/38. However, FDL-labeled PGC were not observed in almost all the 2L-13 antibody-injected tadpoles, although a similar number of labeled somatic cells were always present. As 2L-13 antibody specifically reacts with XVLG1 protein in the embryos by immunoblotting, the present results suggest that the antibody perturbed the function of XVLG1 protein in the pPGC, resulting in failure of PGC differentiation at the tadpole stage.
为了了解非洲爪蟾维甲酸同源物（非洲爪蟾类维甲酸基因1，XVLG1）蛋白在生殖系细胞中的作用，研究人员尝试用抗维甲酸抗体2L-13干扰该蛋白的功能。将2L-13抗体或对照抗体与谱系示踪剂（异硫氰酸荧光素-葡聚糖-赖氨酸，FDL）显微注射到含有非洲爪蟾32细胞胚胎生殖质的单个植物性卵裂球中，这些卵裂球的后代注定会分化为少量原始生殖细胞（PGC）和大量体细胞，在蝌蚪阶段主要是内胚层组织的体细胞。在37/38期之前，未观察到抗体注射对FDL标记的推定PGC（pPGC）有显著影响。然而，在几乎所有注射了2L-13抗体的蝌蚪中都未观察到FDL标记的PGC，尽管总是存在数量相似的标记体细胞。由于2L-13抗体通过免疫印迹法与胚胎中的XVLG1蛋白特异性反应，目前的结果表明该抗体干扰了pPGC中XVLG1蛋白的功能，导致蝌蚪阶段PGC分化失败。
