Lengwinat T, Meyer H H
Institute for Zoo Biology and Wildlife Research, Berlin, Germany.
Anim Reprod Sci. 1996 Dec 2;45(1-2):103-7. doi: 10.1016/s0378-4320(96)01573-4.
These data provide direct evidence for slow proliferation activity in pre-implantation roe deer trophoblasts and even minor growth of the embryoblast. The blastocyst grows very slowly from August to January. Incorporation of BrdU into the nuclei during a period of 4 h was used to determine the proliferation of the embryonic cells in vitro. The results show for the first time the DNA synthesis in trophoblast cells of roe deer blastocysts from October to December. During the period of October to November the incorporation of BrdU is weak, and in December there is a duplication of DNA metabolism.
这些数据为植入前狍滋养层细胞的缓慢增殖活动以及胚泡甚至极轻微的生长提供了直接证据。从8月到1月，囊胚生长非常缓慢。在4小时内将BrdU掺入细胞核中用于确定体外胚胎细胞的增殖。结果首次显示了10月至12月狍囊胚滋养层细胞中的DNA合成。在10月至11月期间，BrdU的掺入较弱，而在12月存在DNA代谢的复制。
