Livingston A
Cell Tissue Res. 1977 Aug 26;182(3):401-7. doi: 10.1007/BF00219774.
Tannic acid in glutaraldehyde was used to stain microtubules in myelinated and unmyelinated axons of rat sciatic nerve. In the majority of areas the tannic acid failed to penetrate the unmyelinated axons whilst penetrating neighbouring myelinated axons, suggesting a difference in the ability of the two types of nerves to exclude tannic acid. Where tannic acid had penetrated the unmyelinated axons the 13 protofilament substructure and size of the microtubules appeared identical to those seen in the myelinated axons.
戊二醛中的单宁酸被用于对大鼠坐骨神经的有髓和无髓轴突中的微管进行染色。在大多数区域，单宁酸无法穿透无髓轴突，却能穿透相邻的有髓轴突，这表明两种类型的神经在排除单宁酸的能力上存在差异。在单宁酸穿透无髓轴突的地方，微管的13原纤维亚结构和大小与在有髓轴突中看到的相同。
