落叶松阿拉伯半乳聚糖免疫分析方法的建立及其在大鼠血液中落叶松阿拉伯半乳聚糖检测中的应用。

Development of an immunoassay for larch arabinogalactan and its use in the detection of larch arabinogalactan in rat blood.

作者信息

Groman E V, Gou D

机构信息

Advanced Magnetics, Inc., Cambridge, MA 02138, USA.

出版信息

Carbohydr Res. 1997 Jun 11;301(1-2):69-76. doi: 10.1016/s0008-6215(97)00077-3.

DOI:10.1016/s0008-6215(97)00077-3

PMID:9228740

Abstract

We describe a sensitive and convenient immunoassay for larch arabinogalactan and demonstrate its specificity for larch arabinogalactan. Anti-larch arabinogalactan antiserum is about 10(4) and 10(6) times more selective for detecting larch arabinogalactan than antiserum binds to branch terminal disaccharides consisting of the terminal beta-D-galactosyl residue and the penultimate branch (1-->6)-beta-D-galactosyl residue. It does not bind L-arabinose. The sensitivity of the assay for larch arabinogalactan is less than 0.1 microgram/mL. The application of the assay for measuring arabinogalactan pharmacokinetics in rat blood is illustrated.

摘要

我们描述了一种用于检测落叶松阿拉伯半乳聚糖的灵敏且便捷的免疫测定法，并证明了其对落叶松阿拉伯半乳聚糖的特异性。抗落叶松阿拉伯半乳聚糖抗血清在检测落叶松阿拉伯半乳聚糖方面的选择性比与由末端β-D-半乳糖基残基和倒数第二个分支（1→6）-β-D-半乳糖基残基组成的分支末端二糖结合的抗血清高约10^4倍和10^6倍。它不与L-阿拉伯糖结合。该落叶松阿拉伯半乳聚糖测定法的灵敏度低于0.1微克/毫升。文中举例说明了该测定法在测量大鼠血液中阿拉伯半乳聚糖药代动力学方面的应用。