Amitrano A A, Saenz D A, Ramos E H
Departamento de Bioquímica, Facultad de Medicina, Universidad de Buenos Aires-CONICET, Argentina.
FEMS Microbiol Lett. 1997 Jun 15;151(2):131-3. doi: 10.1111/j.1574-6968.1997.tb12560.x.
General amino acid permease (GAP1) activity was evaluated in adenylate cyclase-deficient Saccharomyces cerevisiae to determine the effect of cAMP levels on GAP1 activity. Lowering cAMP concentrations in the culture media led to a decrease in the initial rates of L-citrulline uptake. Kinetics of the amino acid transport system showed a partial loss of transport capacity, with no apparent modifications in permease affinity.
在缺乏腺苷酸环化酶的酿酒酵母中评估了通用氨基酸通透酶（GAP1）的活性，以确定环磷酸腺苷（cAMP）水平对GAP1活性的影响。降低培养基中环磷酸腺苷的浓度会导致L-瓜氨酸摄取初始速率的下降。氨基酸转运系统的动力学显示转运能力部分丧失，通透酶亲和力无明显改变。
