来自美国和以色列的海豚链球菌菌株的16S rDNA和整个rRNA基因的限制性片段长度多态性（核糖体分型）
Restriction fragment length polymorphisms of 16S rDNA and of whole rRNA genes (ribotyping) of Streptococcus iniae strains from the United States and Israel.
作者信息
Eldar A, Lawhon S, Frelier P F, Assenta L, Simpson B R, Varner P W, Bercovier H
机构信息
Department of Clinical Microbiology, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
出版信息
FEMS Microbiol Lett. 1997 Jun 15;151(2):155-62. doi: 10.1111/j.1574-6968.1997.tb12564.x.
Streptococcus iniae (junior synonym S. shiloi) isolated from tilapia and trout in Israel and in the United States were subtyped by restriction length polymorphism (RFLP) based on PCR amplified 16S rDNA and by ribotyping. 16S rDNA RFLP discriminated between S. iniae and other fish pathogens but not between S. iniae strains. HindIII and EcoRI ribotypes of S. iniae discriminated American from Israeli strains rejecting the possibility of an epidemiological link between S. iniae infections in the two countries. Israeli strains isolated from tilapia and trout could not be completely differentiated. The S. iniae ATCC 29178T (T = Type strain) strain, isolated from a freshwater dolphin belonged to a ribotype different from those of all the fish isolates.
从以色列和美国的罗非鱼和鳟鱼中分离出的海豚链球菌（次同义词希洛伊链球菌），通过基于聚合酶链反应（PCR）扩增的16S核糖体DNA的限制性片段长度多态性（RFLP）以及核糖体分型进行亚型分析。16S rDNA RFLP能够区分海豚链球菌与其他鱼类病原体，但无法区分海豚链球菌菌株。海豚链球菌的HindIII和EcoRI核糖体分型能够区分美国菌株和以色列菌株，排除了两国海豚链球菌感染之间存在流行病学关联的可能性。从罗非鱼和鳟鱼中分离出的以色列菌株无法完全区分。从淡水海豚中分离出的海豚链球菌ATCC 29178T菌株（T = 模式菌株）属于一种与所有鱼类分离株不同的核糖体分型。
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