海豚链球菌16S - 23S核糖体DNA基因间隔区特异性巢式寡核苷酸PCR引物的研制。
Development of specific nested oligonucleotide PCR primers for the Streptococcus iniae 16S-23S ribosomal DNA intergenic spacer.
作者信息
Berridge B R, Fuller J D, de Azavedo J, Low D E, Bercovier H, Frelier P F
机构信息
Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station, Texas 77843, USA.
出版信息
J Clin Microbiol. 1998 Sep;36(9):2778-81. doi: 10.1128/JCM.36.9.2778-2781.1998.
Abstract
Streptococcus iniae is a cause of septicemia, meningoencephalitis, and death in farmed fish and of cellulitis in human beings. A set of nested oligonucleotide PCR primers that specifically amplified a 373-bp subunit from a variety of clinical isolates from farmed fish and human patients were constructed from a 524-bp consensus sequence of the S. iniae 16S-23S ribosomal DNA intergenic spacer.
摘要
海豚链球菌是养殖鱼类败血症、脑膜脑炎和死亡的病因，也是人类蜂窝织炎的病因。根据海豚链球菌16S - 23S核糖体DNA基因间隔区524 bp的共有序列构建了一组巢式寡核苷酸PCR引物，该引物能从养殖鱼类和人类患者的多种临床分离株中特异性扩增出一个373 bp的亚基。
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