Abdulmawjood A, Weiss R, Lämmler C
Bakteriologie und Hygiene der Milch, Justus-Liebig Universität Giessen, Germany.
Res Vet Sci. 1998 Jul-Aug;65(1):85-6. doi: 10.1016/s0034-5288(98)90033-9.
Streptococcus porcinus reference strains and routine isolates belonging to Lancefield's serogroup E, P, U and V and to various serotypes of serogroup E were examined for their 16S ribosomal DNA fingerprint pattern. Oligonucleotide primers complementary to 16S rRNA genes were used to amplify gene fragments by polymerase chain reaction from genomic DNA. The amplified 1450 bp fragment was subsequently digested with the restriction enzyme BpiI resulting in two fragments with a size of approximately 1250 bp and 200 bp. All 45 S porcinus investigated in the present study could be identified on the basis of this characteristic 16S rDNA fingerprint pattern and clearly differentiated from 16 control strains of various species and serogroups of genus Streptococcus. The present results demonstrate the potential application of 16S rDNA analysis for identification of S porcinus, a species which might express various group- and type-specific antigens.
对属于兰斯菲尔德血清群E、P、U和V以及血清群E各种血清型的猪链球菌参考菌株和常规分离株进行了16S核糖体DNA指纹图谱分析。使用与16S rRNA基因互补的寡核苷酸引物，通过聚合酶链反应从基因组DNA中扩增基因片段。随后，用限制性内切酶BpiI消化扩增的1450 bp片段，产生两个大小约为1250 bp和200 bp的片段。根据这一特征性的16S rDNA指纹图谱，本研究中调查的所有45株猪链球菌均可被鉴定，并与链球菌属不同种和血清群的16株对照菌株明显区分开来。目前的结果表明，16S rDNA分析在鉴定猪链球菌方面具有潜在应用价值，猪链球菌可能表达各种群特异性和型特异性抗原。
