Zoorob G K, Caruso J A
Department of Chemistry, University of Cincinnati, OH 45221, USA.
J Chromatogr A. 1997 Jun 27;773(1-2):157-62. doi: 10.1016/s0021-9673(97)00074-5.
High-performance liquid chromatography (HPLC) coupled to inductively coupled plasma mass spectrometry (ICP-MS) was employed for the separation and detection of chromium species in azo dyes, Acid blue 158 and Acid Blue 193; mainly Cr(III) and Cr(VI). The dyes were first analyzed for total metal content using ICP-MS and their stability in solution was studied by measuring their absorbance through a range of pH values. Then an isocratic chromatographic method employing reversed-phase liquid chromatography with mass spectrometric detection was developed. Applying this method to the separation of these dyes, the absolute detection limits of Acid Blue 158 and 193 were 1 and 5 ng respectively. Additionally, Acid Blue 158 did not contain any chromium species. On the other hand, Acid Blue 193 contained uncomplexed and potentially bioavailable Cr(III). Acid Blue 193 did not have any toxic Cr(VI) present in the samples.
采用高效液相色谱（HPLC）与电感耦合等离子体质谱（ICP-MS）联用技术，对偶氮染料酸性蓝158和酸性蓝193中的铬形态（主要是Cr(III)和Cr(VI)）进行分离和检测。首先使用ICP-MS分析染料中的总金属含量，并通过测量一系列pH值下的吸光度来研究它们在溶液中的稳定性。然后开发了一种采用反相液相色谱-质谱检测的等度色谱方法。将该方法应用于这些染料的分离，酸性蓝158和193的绝对检测限分别为1 ng和5 ng。此外，酸性蓝158不含任何铬形态。另一方面，酸性蓝193含有未络合的、具有潜在生物可利用性的Cr(III)。样品中的酸性蓝193未检测到任何有毒的Cr(VI)。
