Ito A, Ito M, Eom K S, Chung W C, Chen C C, Ma L, Endo S, Fan P C
Department of Parasitology, Gifu University School of Medicine, Japan.
Int J Parasitol. 1997 Jun;27(6):631-3. doi: 10.1016/s0020-7519(97)00017-9.
In vitro hatched (but not activated) oncospheres of Asian Taenia obtained in Korea and Taiwan, prepared by the sodium hypochlorite method, rinsed with sterile PBS several times and adjusted to 5 x 10(4)/0.5 ml PBS, were injected intraperitoneally or subcutaneously into male or female scid mice of 3 different strains. When these scid mice were sacrificed 4 months later, the females harboured fully developed cysticerci either in the peritoneal cavity or under the back skin, whereas males did not. All cysticerci from the peritoneal cavity were easily recovered by rinsing the abdomen with PBS. Although most cysticerci recovered from pig liver usually become calcified within 1-2 months, in female scid mice they all increased in size and were viable. Intraperitoneal (i.p.) injection of in vitro hatched oncospheres is recommended for easier recovery of Asian Taenia metacestodes in laboratory animals.
在韩国和台湾获得的亚洲带绦虫的体外孵化（但未激活）六钩蚴，采用次氯酸钠法制备，用无菌磷酸盐缓冲盐水（PBS）冲洗数次并调整至5×10⁴/0.5 ml PBS，经腹腔或皮下注射到3种不同品系的雄性或雌性重度联合免疫缺陷（scid）小鼠体内。4个月后处死这些scid小鼠时，雌性小鼠在腹腔或背部皮肤下有完全发育的囊尾蚴，而雄性小鼠则没有。通过用PBS冲洗腹部可轻松回收腹腔内的所有囊尾蚴。虽然从猪肝中回收的大多数囊尾蚴通常在1 - 2个月内钙化，但在雌性scid小鼠中它们都增大了尺寸且存活。建议腹腔注射体外孵化的六钩蚴，以便在实验动物中更轻松地回收亚洲带绦虫的中绦期幼虫。
