Pazlarová J, Demnerová K, Macková M, Burkhard J
Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague, Czech Republic.
Lett Appl Microbiol. 1997 May;24(5):334-6. doi: 10.1046/j.1472-765x.1997.00075.x.
Several aerobic co-cultures capable of co-metabolising polychlorinated biphenyls (PCBs) were acquired by cultivation on biphenyls (BP). The source of micro-organisms was PCB-contaminated soil taken from various sites in the Czech Republic. Several bacterial strains (Gram-negative rods) were isolated, and their capacity to degrade Delor 103 (a PCB mixture containing di- to hexachlorobiphenyls) was analysed. This study was focused on co-culture 319 and isolate 2. The growth parameters of both those cultures were studied on BP; for isolate number 2 the specific growth rate mu = 0.122 (h-1) was calculated. The degradation of the individual congeners was estimated and resulted in more than 50% of the degradation of nearly all congeners during a 2-week experiment. Toxicity of Delor 103 on the vitality of the cells was followed by using viable plate count. The viability of the tested strain was preserved in the 100 times higher Delor 103 concentration compared with conditions in degradation experiments.
通过在联苯（BP）上培养，获得了几种能够共代谢多氯联苯（PCBs）的需氧共培养物。微生物来源是取自捷克共和国不同地点的受多氯联苯污染的土壤。分离出了几种细菌菌株（革兰氏阴性杆菌），并分析了它们降解Delor 103（一种含有二至六氯联苯的多氯联苯混合物）的能力。本研究重点关注共培养物319和分离物2。在联苯上研究了这两种培养物的生长参数；对于分离物2，计算出比生长速率μ = 0.122（h-1）。估计了各个同系物的降解情况，在为期2周的实验中，几乎所有同系物的降解率都超过了50%。通过活菌平板计数追踪Delor 103对细胞活力的毒性。与降解实验条件相比，在浓度高出100倍的Delor 103中，受试菌株的活力得以保留。
