Wooder M F, Wright A S, King L J
Chem Biol Interact. 1977 Oct;19(1):25-46. doi: 10.1016/0009-2797(77)90040-0.
Twenty male CFE rats were exposed to atmospheres containing 0.064 microgram/l of [Me-14C] dichlorvos (113 Ci/mol) for 12 h. Analysis of the DNA and RNA from the total soft tissues of these rats revealed no methylation of the N7 atom of guanine moieties. The limits of detection of methylation were one methyl group per 6.0 X 10(11) and per 2 X 10(9) nucleotide units for DNA and RNA, respectively. Only 0.000001% of the administered dose would have needed to react with DNA in order to produce detectable methylation of this macromolecule. The exposure period employed in this study (12 h) constituted a significant fraction of the half-life of 7-methylguanine moieties in DNA (3 days). On the basis of this information and the extremely rapid metabolism of dichlorvos in a wide range of mammalian tissues and species it was concluded that dichlorvos does not methylate the nucleic acids of mammalian tissues when it is inhaled continuously at practical use concentrations.
将20只雄性CFE大鼠暴露于含有0.064微克/升[甲基-14C]敌敌畏（113居里/摩尔）的大气中12小时。对这些大鼠全软组织的DNA和RNA分析显示，鸟嘌呤部分的N7原子没有甲基化。DNA和RNA甲基化的检测限分别为每6.0×10¹¹和每2×10⁹个核苷酸单位一个甲基。仅需0.000001%的给药剂量与DNA反应，就能产生该大分子可检测到的甲基化。本研究采用的暴露时间（12小时）占DNA中7-甲基鸟嘌呤部分半衰期（3天）的很大一部分。基于这些信息以及敌敌畏在广泛的哺乳动物组织和物种中极其快速的代谢，得出结论：当以实际使用浓度持续吸入时，敌敌畏不会使哺乳动物组织的核酸甲基化。
