Poo J L, Estanes A, Pedraza-Chaverrí J, Cruz C, Pérez C, Huberman A, Uribe M
Departamentos de Gastroenterología, Instituto Nacional de la Nutrición, Salvador Zubirán, México, D.F.
Rev Invest Clin. 1997 Jan-Feb;49(1):15-23.
Bile duct obstruction was induced in 127 male Wistar rats, and renal function, hemodynamic, biochemical and liver histology were evaluated at weeks 1, 2, 3 and 4 after complete bile duct obstruction; the data were compared to that in 30 control rats.
Portal pressure significantly increased at week 1 (11.7 +/- 1.5. vs. 7.8 +/- 1.5 mmHg, p < 0.05) while the mean arterial pressure remained stable until week 4 when a slight decrease was observed (91.3 +/- 6.6 vs. 96.1 +/- 8.6 mmHg in control rats). A significant decrease in urinary sodium excretion was observed at week 1 (1.1 +/- 0.5 mEq/24 h) compared to control rats (2.3 +/- 0.6 mEq/24 h). In addition, hyperreninemia was observed at week 1 (5.1 +/- 0.2 vs. 2.4 +/- 1.3 ng Ang l/mL/h, p < 0.05) and hyperaldosteronism at week 2 (103 +/- 46 vs. 25.1 +/- 8.8 ng/24 h, p < 0.05) compared to control rats.
A temporal relationship between the beginning of portal hypertension and a decrease of renal sodium excretion, hyperreninemia and hyperaldosteronism was observed in bile duct ligated rats. This experimental model could be used to evaluate the effects of new drugs to prevent biliary cirrhosis including the abnormalities in the renal handling of sodium.
1）评估胆管梗阻诱导的大鼠生化、肾脏、组织学以及内脏和全身血流动力学异常；2）研究门静脉高压起始、尿钠排泄减少与肾素-血管紧张素系统激活之间的时间关系。
对127只雄性Wistar大鼠进行胆管梗阻诱导，并在完全胆管梗阻后第1、2、3和4周评估其肾功能、血流动力学、生化指标和肝脏组织学；将数据与30只对照大鼠的数据进行比较。
门静脉压力在第1周显著升高（11.7±1.5对7.8±1.5 mmHg，p<0.05），而平均动脉压直到第4周保持稳定，此时观察到轻微下降（对照大鼠为96.1±8.6 mmHg，现为91.3±6.6 mmHg）。与对照大鼠（2.3±0.6 mEq/24 h）相比，第1周观察到尿钠排泄显著减少（1.1±0.5 mEq/24 h）。此外，与对照大鼠相比，第1周观察到高肾素血症（5.1±0.2对2.4±1.3 ng Ang l/mL/h，p<0.05），第2周观察到高醛固酮血症（103±46对25.1±8.8 ng/24 h，p<0.05）。
在胆管结扎大鼠中观察到门静脉高压起始与肾钠排泄减少、高肾素血症和高醛固酮血症之间的时间关系。该实验模型可用于评估预防胆汁性肝硬化新药的效果，包括钠的肾脏处理异常。
