通过化学交联对外膜线粒体中细胞死亡蛋白的研究。
The study of cell-death proteins in the outer mitochondrial membrane by chemical cross-linking.
作者信息
Ali S T, Coggins J R, Jacobs H T
机构信息
Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, Scotland, U.K.
出版信息
Biochem J. 1997 Jul 15;325 ( Pt 2)(Pt 2):321-4. doi: 10.1042/bj3250321.
Abstract
Chemical cross-linking was used to study the interactions of the anti-cell-death protein Bcl2 with other proteins in the outer mitochondrial membrane. Cross-linking of mitochondrial surface proteins produced a large Bcl2-containing complex (>200 kDa), and a Bcl2-derived peptide was shown to cross-link specifically with a mitochondrial protein identified by immunoblotting as Raf-1 kinase.
摘要
化学交联法被用于研究抗细胞死亡蛋白Bcl2与线粒体外膜中其他蛋白质的相互作用。线粒体表面蛋白的交联产生了一个大的含Bcl2复合物（>200 kDa），并且一个源自Bcl2的肽段被证明能与通过免疫印迹鉴定为Raf-1激酶的线粒体蛋白特异性交联。
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