Weinberger H, Lichte E, Griesinger C, Kutscher B
Institute of Organic Chemistry, J.W. Goethe-University of Frankfurt, Frankfurt/Main, Germany.
Arch Pharm (Weinheim). 1997 Apr;330(4):109-11. doi: 10.1002/ardp.19973300406.
Peptides from small combinatorial libraries, covalently attached to polymeric TentaGel beads, can be directly sequenced using amino acid analysis. For libraries with restricted diversity, generated by the split-mix synthesis method, the amino acids on a selected single bead identified by pre-column derivatization with o-phthaldialdehyde (OPA) correlate directly with the sequence of a given peptide. This is shown on a tripeptide (343 different compounds) and a tetrapeptide (4096 different compounds) library. This method allows for rapid peptide sequence determination without relying on complex encoding strategies.
来自小型组合文库、共价连接到聚合物TentaGel珠上的肽段，可以使用氨基酸分析直接进行测序。对于通过分割混合合成法产生的具有有限多样性的文库，用邻苯二甲醛（OPA）进行柱前衍生化鉴定出的单个选定珠子上的氨基酸，与给定肽段的序列直接相关。这在一个三肽（343种不同化合物）文库和一个四肽（4096种不同化合物）文库中得到了证明。这种方法无需依赖复杂的编码策略就能快速确定肽段序列。
