López-Pérez Paula M, Grimsey Elizabeth, Bourne Luc, Mikut Ralf, Hilpert Kai
TiKa Diagnostics LtdLondon, UK.
Institute for Infection and Immunity, St. George's University of LondonLondon, UK.
Front Chem. 2017 Apr 12;5:25. doi: 10.3389/fchem.2017.00025. eCollection 2017.
Peptide arrays on cellulose are a powerful tool to investigate peptide interactions with a number of different molecules, for examples antibodies, receptors or enzymes. Such peptide arrays can also be used to study interactions with whole cells. In this review, we focus on the interaction of small antimicrobial peptides with bacteria. Antimicrobial peptides (AMPs) can kill multidrug-resistant (MDR) human pathogenic bacteria and therefore could be next generation antibiotics targeting MDR bacteria. We describe the screen and the result of different optimization strategies of peptides cleaved from the membrane. In addition, screening of antibacterial activity of peptides that are tethered to the surface is discussed. Surface-active peptides can be used to protect surfaces from bacterial infections, for example implants.
纤维素上的肽阵列是研究肽与多种不同分子（如抗体、受体或酶）相互作用的有力工具。此类肽阵列也可用于研究与完整细胞的相互作用。在本综述中，我们聚焦于小型抗菌肽与细菌的相互作用。抗菌肽（AMPs）能够杀死多重耐药（MDR）的人类致病细菌，因此有望成为针对MDR细菌的下一代抗生素。我们描述了从膜上切割下来的肽的筛选过程以及不同优化策略的结果。此外，还讨论了连接在表面的肽的抗菌活性筛选。表面活性肽可用于保护表面免受细菌感染，例如植入物。
