Akaki C, Simazu M, Baba T, Tsuji S, Kodama H, Mukamoto M, Kajikawa T
Department of Veterinary Immunology, College of Agriculture, Osaka Prefecture University, Japan.
Zentralbl Veterinarmed B. 1997 Jun;44(4):199-206. doi: 10.1111/j.1439-0450.1997.tb00965.x.
This study was designed to evaluate the significance of the Harderian gland (HG) in the chicken IgA-production system by investigating the influence of HG on the increase of surface IgA (sIgA)-positive cells in other lymphoid organs and also the possibility of migration of HG lymphocytes into other organs. Non-contact culture of splenic or caecal tonsil (CT) lymphocytes with HG whole cells did not enhance the expression of sIgA. The experiment of HG lymphocyte transfer showed that the transferred HG lymphocytes migrated mainly into the CT in the 3-week-old recipient, and mainly into the CT and bursa of Fabricius (BF) in the 6-week-old recipient. It was also found that the transferred sIgA-positive HG lymphocytes migrated selectively into CT in both 3-week-old and 6-week-old recipients. These results indicated that chicken HG plays a central role not only in governing the local immunity in the eyes and respiratory tract but also as an organ which supplies precursory IgA-producing cells involved in local immunity in the intestine.
本研究旨在通过研究哈德氏腺（HG）对其他淋巴器官中表面IgA（sIgA）阳性细胞增加的影响以及HG淋巴细胞迁移到其他器官的可能性，来评估哈德氏腺在鸡IgA产生系统中的重要性。脾或盲肠扁桃体（CT）淋巴细胞与HG全细胞的非接触培养并未增强sIgA的表达。HG淋巴细胞转移实验表明，转移的HG淋巴细胞在3周龄受体中主要迁移到CT，在6周龄受体中主要迁移到CT和法氏囊（BF）。还发现，转移的sIgA阳性HG淋巴细胞在3周龄和6周龄受体中均选择性地迁移到CT。这些结果表明，鸡HG不仅在控制眼和呼吸道的局部免疫中起核心作用，而且作为一个为肠道局部免疫提供产生IgA前体细胞的器官发挥作用。
