Rabinowitz J L, Staeffen J, Aumonier P, Ballan P, Ferrer J, Terme R, Series C, Myerson R M
Clin Chem. 1977 Dec;23(12):2202-6.
We describe a new, more efficient, and more reproducible method for determination of octanoate in serum. This method involves ethanol extraction, followed immediately by alkali addition before concentration of the extract. The concentrate is made acidic only before it is to be steam distilled (in a special all-glass apparatus with an alkali trap). The material is acidified again just before separation by gas-liquid chromatography. The yield is 89-107%. When assayed by mass spectrometry, only octanoate was found in the fraction from chromatography. Previous methods yielded only 30-55% of the expected octanoate value and the recovered materials showed impurities by mass spectrometry. Octanoate concentrations were determined in the serum of 24 fasting controls and that of 85 fasting cirrhotic patients, of whom 50 had encephalopathy. Concentrations in arterial and venous blood were significantly higher in cirrhotic patients in coma than in those not in coma, and arterial concentrations were statistically higher than venous concentrations in the cirrhotic patients.
我们描述了一种测定血清中辛酸的新方法，该方法更高效、更具可重复性。此方法包括乙醇萃取，萃取后在浓缩提取物之前立即加入碱。浓缩物仅在进行蒸汽蒸馏（在带有碱阱的特殊全玻璃装置中）之前酸化。在通过气液色谱分离之前，物料再次酸化。产率为89 - 107%。通过质谱分析时，色谱馏分中仅发现了辛酸。以前的方法仅得到预期辛酸值的30 - 55%，并且回收的物质通过质谱显示有杂质。测定了24名空腹对照者以及85名空腹肝硬化患者（其中50名患有肝性脑病）的血清中的辛酸浓度。昏迷的肝硬化患者动脉血和静脉血中的浓度显著高于未昏迷者，并且肝硬化患者的动脉血浓度在统计学上高于静脉血浓度。
