Staeffen J, Rabinowitz J L, Aumonier P, Ballan P, Ferrer J, Terme R, Seriès C, Myerson R M
Nouv Presse Med. 1979 May 5;8(20):1663-6.
A new and sensitive method for determination of octanoate in serum by gas-liquid chromatography is described. It was validated by mass spectrometry. Octanoate concentrations were determined in the serum of 61 fasting cirrhotic patients of which 47 also had hepatic encephalopathy. Concentrations in arterial and venous blood were higher in cirrhotic patients with encephalopathy than in those without and higher in the latter than in controls. Arterial concentrations were higher than venous concentrations and octanoate and ammonia varied independently. A predominant endogenous origin is likely. Data obtained from studies using palmitic acid labeled at different loci suggest that recovered serum octanoate was formed mostly by incomplete oxidation of long chain fatty acids. Sodium octanoate infusion to rhesus monkeys studied polygraphically induces a temporary coma.
本文描述了一种通过气液色谱法测定血清中辛酸的新的灵敏方法。该方法已通过质谱法验证。测定了61例空腹肝硬化患者血清中的辛酸浓度，其中47例还患有肝性脑病。有脑病的肝硬化患者动脉血和静脉血中的辛酸浓度高于无脑病的患者，且后者高于对照组。动脉血浓度高于静脉血浓度，辛酸和氨的变化相互独立。辛酸很可能主要来源于内源性。使用在不同位点标记的棕榈酸进行研究获得的数据表明，回收的血清辛酸大多是由长链脂肪酸不完全氧化形成的。对恒河猴静脉输注辛酸钠并进行多导记录研究发现，这会导致暂时昏迷。
