Nandi S, Subudhi P K, Senadhira D, Manigbas N L, Sen-Mandi S, Huang N
Division of Plant Breeding, Genetics and Biochemistry, International Rice Research Institute, Manila, Philippines.
Mol Gen Genet. 1997 Jun;255(1):1-8. doi: 10.1007/s004380050468.
By combining the amplified fragment length polymorphism (AFLP) technique with selective genotyping, we constructed a linkage map for rice and assigned each linkage group to a corresponding chromosome. The AFLP map, consisting of 202 AFLP markers, was generated from 74 recombinant inbred lines (RIL) which were selected from both extremes of the population (250 lines) with respect to the response to complete submergence. Map length was 1756 cM, with an average interval size of 8.5 cM. To assign linkage groups to chromosomes, we used 50 previously mapped AFLP markers as anchor markers distributed over the 12 chromosomes. Other AFLP markers were then assigned to specific chromosomes based on their linkage to anchor markers. This AFLP map is equivalent to the RFLP/AFLP map constructed previously as the anchors were in the same order in both maps. Furthermore, tests with two restriction fragment length polymorphism (RFLP) markers and two sequence-tagged site (STS) markers showed that they mapped in the expected positions. Using this AFLP map, a major gene for submergence tolerance was localized on chromosome 9. Quantitative trait loci (QTL) associated with submergence tolerance were detected on chromosomes 6, 7, 11, and 12. We conclude that the combination of AFLP mapping and selective genotyping provides a much faster and easier approach to QTL identification than the use of RFLP markers.
通过将扩增片段长度多态性（AFLP）技术与选择性基因分型相结合，我们构建了水稻的连锁图谱，并将每个连锁群对应到相应的染色体上。该AFLP图谱由202个AFLP标记组成，来自74个重组自交系（RIL），这些重组自交系是从250个品系的群体两端选取的，选取依据是对完全淹水的响应。图谱长度为1756厘摩，平均间隔大小为8.5厘摩。为了将连锁群对应到染色体上，我们使用了50个先前定位的AFLP标记作为锚定标记，这些标记分布在12条染色体上。然后根据其他AFLP标记与锚定标记的连锁关系将它们定位到特定染色体上。这个AFLP图谱与先前构建的RFLP/AFLP图谱相当，因为两个图谱中的锚定标记顺序相同。此外，用两个限制性片段长度多态性（RFLP）标记和两个序列标签位点（STS）标记进行的测试表明，它们定位在预期位置。利用这个AFLP图谱，一个耐淹主基因被定位到9号染色体上。在6号、7号、11号和12号染色体上检测到了与耐淹性相关的数量性状位点（QTL）。我们得出结论，与使用RFLP标记相比，AFLP作图和选择性基因分型相结合为QTL鉴定提供了一种更快、更简便的方法。
