Xu K, Xu X, Ronald P C, Mackill D J
Department of Agronomy and Range Science, University of California, Davis 95616, USA.
Mol Gen Genet. 2000 May;263(4):681-9. doi: 10.1007/s004380051217.
Resistance to submergence stress is an important breeding objective in areas where rice cultivars are subjected to complete inundation for a week or more. The present study was conducted to develop a high-resolution map of the region surrounding the submergence tolerance gene Sub1 in rice, which derives from the Indian cultivar FR13A. Submergence screening of 8-day-old plants of F3 families kept for 14 days submerged in 60 cm of water allowed an accurate classification of Sub1 phenotypes. Bulked segregant analysis was used to identify AFLP markers linked to Sub1. A population of 2950 F2 plants segregating for Sub1 was screened with two RFLP markers flanking the Sub1 locus, 2.4 and 4.9 cM away. Submergence tolerance was measured in the recombinant plants, and AFLP markers closely linked to Sub1 were mapped. Two AFLP markers cosegregated with Sub1 in this large population, and other markers were localized within 0.2 cM of Sub1. The high-resolution map should serve as the basis for map-based cloning of this important locus, as it will permit the identification of BAC clones spanning the region.
在水稻品种遭受一周或更长时间完全淹没的地区，耐淹胁迫是一个重要的育种目标。本研究旨在绘制水稻中耐淹基因Sub1周围区域的高分辨率图谱，该基因源自印度品种FR13A。对F3家系8日龄植株进行淹水筛选，将其置于60厘米深的水中淹没14天，从而能够准确分类Sub1表型。利用混合分离群体分析法鉴定与Sub1连锁的AFLP标记。用位于Sub1基因座两侧、距离分别为2.4和4.9厘摩的两个RFLP标记对2950株分离Sub1的F2植株群体进行筛选。在重组植株中测定耐淹性，并对与Sub1紧密连锁的AFLP标记进行定位。在这个大群体中，有两个AFLP标记与Sub1共分离，其他标记位于距离Sub1 0.2厘摩以内。该高分辨率图谱应为该重要基因座的图位克隆奠定基础，因为它将有助于鉴定跨越该区域的BAC克隆。
