Luo Y, Koop H U
Laboratory of Cell Biology and Cell Culture, Ludwig-Maximilians-Universität, München, Germany.
Planta. 1997;202(3):387-96. doi: 10.1007/s004250050141.
Immature zygotic embryos of six ecotypes (Nd-0, Ler, C24, Col-0, Nossen, Ws-2) of Arabidopsis thaliana (L.) Heynh. were cultured in vitro. The same ecotypes, except Nossen, were used for studies on leaf protoplast culture. Experimental conditions for the induction of somatic embryos were established in both culture systems. In the case of immature zygotic embryos, the parameters investigated were the influence of developmental stage of the explant, the ecotypes used, and various concentrations and combinations of growth regulatory substances (phytohormones). In the ecotype Ler, structures were discovered which were very similar to those found in the early stages of zygotic embryo-genesis: globular structures at the end of a suspensor-like single file of cells were frequently observed. In the case of leaf protoplasts, high efficiencies of colony formation and plant regeneration occurred in Ws-2 and C24. A novel type of cell division pattern was found in Col-0 and C24, again highly reminiscent of the early division patterns in zygotic embryos. Similarities and differences between zygotic and somatic embryogenesis are discussed.
对拟南芥（L.）海恩（Heynh.）六个生态型（Nd-0、Ler、C24、Col-0、Nossen、Ws-2）的未成熟合子胚进行了离体培养。除Nossen外，相同的生态型用于叶片原生质体培养研究。在两种培养系统中都建立了体细胞胚诱导的实验条件。对于未成熟合子胚，研究的参数包括外植体发育阶段的影响、所用生态型以及生长调节物质（植物激素）的各种浓度和组合。在生态型Ler中，发现了与合子胚发生早期阶段非常相似的结构：经常观察到在类似胚柄的单列细胞末端有球形结构。对于叶片原生质体，Ws-2和C24中出现了高效率的菌落形成和植株再生。在Col-0和C24中发现了一种新型的细胞分裂模式，同样高度让人联想到合子胚的早期分裂模式。讨论了合子胚和体细胞胚发生之间的异同。
